Microplate Removing Proteins from Biological Samples
Editor: Doris Popp
The Protein Crash technique used by the Porvair Sciences P3 microplate is highly effective at removing proteins from biological samples prior to analysis.
The P3 microplate works by denaturing the protein upon addition of solvents such as acetonitrile or methanol. The proteins “crash” or precipitate out of solution to forms large sticky globules that are then trapped on an open-pore structure filter. Underneath this filter is a highly hydrophobic filter which retains all liquids above it. When a vacuum is applied to the P3 microplate, the liquid containing the analytes of interest is efficiently drawn through the filter and into a collection plate below leaving behind unwanted proteins that can interfere with downstream applications.
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