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pH Measurement Yogurt pH: The Science Behind the Taste

From Sakshi Jadhav, Mettler Toledo 3 min Reading Time

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Yogurt, a popular snack for its versatility and health benefits, serves as a practical choice for busy individuals. This article explores how precise pH management during production — crucial for probiotic activity, taste, and shelf life — is achieved through careful monitoring.

pH is a crucial parameter in the production process of yogurt. (Source: ©  Igor Dudchak - stock.adobe.com)
pH is a crucial parameter in the production process of yogurt.
(Source: © Igor Dudchak - stock.adobe.com)

Yogurt is a go-to snack for lots of people these days — not just because it tastes great and can be used in so many ways, but also because it offers real health perks that help keep us feeling good. With so many of us balancing busy work lives, family duties, and everything else in between, yogurt has become an easy, reliable choice for quick breakfasts and lunches.

If you’ve ever wondered how producers make sure yogurt stays just right, especially when it comes to its pH levels, let’s dive into why and how measuring pH is such a crucial part of making your favorite creamy treat.

Yogurt offers some impressive health perks that go beyond just a tasty snack — it can boost your digestion, ramp up your immune system, and help your body absorb nutrients more effectively. What’s behind these benefits? It’s all about those probiotic cultures — live, friendly microorganisms that flourish in your gut. Keeping these tiny health heroes alive and thriving from the moment yogurt is made until it reaches your spoon depends heavily on maintaining the perfect pH level inside the container. Without the right acidity, those probiotics just can’t do their magic.

During yogurt fermentation, the pH of milk drops from approximately 6.7 to 4.6 or lower, fostering an acidic environment that suppresses harmful bacteria while encouraging the growth of beneficial probiotics. The yogurt production begins with homogenization and pasteurization of milk, followed by cooling to an ideal temperature for the starter culture to be introduced. Fermentation starts as live bacterial strains generate lactic acid, lowering the pH. This pH decrease is closely monitored until it achieves the target pH of 4.6 units or less set by the manufacturer. Subsequently, the product is partially cooled below 20 °C, and any desired fruit or flavorings are incorporated. Finally, yogurt is placed into cold storage to halt further acid formation, yielding a delicious final product ready for consumption.

Mettler Toledo’s Inlab Solids Pro-ISM is a cutting-edge sensor designed specifically for accurate and precise pH measurements in yogurt.(Source:  Mettler Toledo)
Mettler Toledo’s Inlab Solids Pro-ISM is a cutting-edge sensor designed specifically for accurate and precise pH measurements in yogurt.
(Source: Mettler Toledo)

The probiotic effectiveness, flavor quality, and shelf stability of yogurt are all influenced by precise pH measurements conducted throughout manufacturing and quality control.

pH measurement is performed with a pH meter and electrode. However, traditional pH sensors encounter challenges measuring yogurt's pH due to several factors. High protein content can cause sensor junction clogging, blocking the reference electrolyte from mixing properly with the sample. Meanwhile, elevated fat levels can foul the sensor by depositing on the glass membrane. Additionally, yogurt's semi-solid nature requires sample preparation, usually by diluting with water, which carries risks of error; direct insertion of the sensor into yogurt may damage the sensor.

Five Tips for the Correct Measurement

Best practices for pH measurement include:

Operating the sensor within its pH range of 1 to 11 units, avoiding exposure to strongly acidic (below pH 1.00) or alkaline (above pH 11.00) solutions.

Cleaning the electrode surface after each measurement to avoid contamination and ensure rapid sensor response.

Using a mild soapy solution followed by a rinse with de-ionized water to thoroughly clean residues after sampling.

Employing fresh buffer solutions in the correct sequence and checking their expiration dates.

Avoiding storage of the electrode dry or in distilled water, as both can harm the pH-sensitive glass membrane and shorten electrode life.

Discover Mettler Toledo's comprehensive guide for conducting accurate pH analysis of yogurt samples. Find the ideal pH measurement system tailored for your specific yogurt testing needs.

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