Figure 1: Principle of 2D gel electrophoresis: Isoelectric focusing (IEF) is performed in the first dimension to separate proteins according to their isoelectric points (see center image). In the second dimension, proteins are separated in SDS electrophoresis (for SDS PAGE, see image on the right) according to their molecular weights.
(Picture: Serva Electrophoresis GmbH, Heidelberg)
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