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Saudi Arabia: Neuroscience Study Sheds Light on the Effects of Lactate on Long-Term Memory

| Editor: Alexander Stark

A genome-wide study examined how lactate regulates long-term memory formation and neuroprotection. Previous studies have shown that lactate stimulates the expression of genes encoding proteins involved in neuronal activity by signaling through N-methyl-D-aspartate (NMDA) receptors. This latest study reveals the extent to which lactate modifies gene expression in cortical neurons and also points to the mechanisms through which lactate modulates brain function.

Michael Margineanu (left), Hanan Mamood (right) and colleagues have identified genes that may assist with developing novel therapeutic targets for neurodegenerative diseases, such as Alzheimer's disease.
Michael Margineanu (left), Hanan Mamood (right) and colleagues have identified genes that may assist with developing novel therapeutic targets for neurodegenerative diseases, such as Alzheimer's disease.
(Source: Helmy H. Alsagaff/ Kaust)

Thuwal /Saudi Arabia — The breakdown of sugar in non-neuronal brain cells, called astrocytes, produces lactate, which gets shuttled to neurons as a source of energy. This lactate not only supports the energy demands of neurons, but also rapidly and transiently activates multiple genes that modulate neuronal activity and regulate brain function.

The team’s genome-wide analysis of gene transcription revealed that lactate exposure triggers the expression of more than 400 genes, some of which are crucial for modifying brain structure and function in response to internal and external influences, known as neuroplasticity. The scientists found that lactate stimulates synaptic activity-dependent genes in the short-term and genes involved in regulating neuronal excitability in the long-term.

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After only an hour of exposure to lactate, 113 genes were differentially expressed compared with controls. Among them were genes known to mediate the NMDA-receptor-dependent response to neuronal activity and those known to be involved in the mitogen-activated protein kinase (MAPK) signaling pathway that regulates neuronal survival.

Selectively blocking NMDA receptors with the inhibitor MK-801 confirmed that 41 percent of the genes modulated by lactate after one hour were activated in an NMDA-receptor dependent manner.

The study also revealed that nicotinamide adenine dinucleotide (NADH), a by-product of the metabolic processing of lactate to pyruvate, was able to regulate the expression of more than 60 % of the genes that were modulated by lactate after one hour. NADH is crucial for the production of adenosine triphosphate (ATP), the major energy currency of the cell. This finding highlights the influence of the cells’ energy production process on neuronal gene expression and signaling.

In addition to identifying genes that are stimulated in a non-NMDA-receptor dependent manner, the study also revealed upregulation in the expression of genes involved in neuronal excitability after six hours of lactate exposure. The identified genes could contribute to the development of novel therapeutic targets for neurodegenerative diseases in which brain energy metabolism is altered, such as Alzheimer’s disease, explains the first author of the paper Michael Margineanu, a Kaust Master’s student.

References: Margineanu, M.B., Mahmood, H., Fiumelli, H. & Magistretti, P.J. L-lactate regulates the expression of plasticity and neuroprotection genes in cortical neurons: a transcriptome analysis. Frontiers in Molecular Neuroscience 11, 375 (2018).|

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