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Mals Detection Multi-Angle Light Scattering Detection

Editor: Alexander Stark

Based on over two decades of practical experience in the area of Laser Light Scattering, Postnova has developed the PN3621 Mals — Multi-Angle Light Scattering detector series. According to the manufacturer, the new model is a new type of high performance Mals detector system with a unique range of specifications.

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The model PN3621 Mals is a new type of high performance Mals detector.
The model PN3621 Mals is a new type of high performance Mals detector.
(Source: Postnova)

Landsberg am Lech/Germany — The precision of molar mass and particles size determination is not only dependent on the number of angles but also on the number of low angles which are available for calculation. Especially, the angles between 0° and 35° are the most important angles for correct and precise molar mass and size determination. Typically, in the past Static Light Scattering has been often limited in use as low angles have not been available or, when available, just do not work properly. The lack of low angles can be tolerated when investigating smaller species, such as proteins or macromolecules where there is no angular intensity dependence. But for larger and more complex samples, such as branched polymers, high molar mass macromolecules, large protein aggregates and nanoparticles, the existence of high quality low angles are critical for precision and accuracy.

Obstacles in Mals Detection

Mals is typically being used to measure larger sample species, such as protein aggregates, high molar mass macromolecules and particles. Because of their size and fragile nature these samples have to be treated in a gentle way to avoid any alteration. Potentially shear forces, chain degradation, conformational changes and deterioration or complete loss of bioactivity can be induced by highly turbulent light scattering cells. Also aggregation, adsorption and sedimentation phenomena can cause trouble inside light scattering cells, especially when they have edges, dead volumes or right angle flow path changes inside. The new avoids all these limitations The flow cell has no right angel entry and outlets which could induce shear forces or cause particles to settle and adsorb.


High Angle Range

The new Postnova PN3621 Mals detector employs full 21 angles and has the broadest angular range of any commercial light scattering instrument available today, the company claims. These 21 angles are crucial for measuring complex particular samples, ensuring a high precision in molecular weight and particle size determination. With 21 angles this detector model can provide data e.g. for high molar mass polymers and large particles. The electronics is based on a true 24 bit resolution AD system and offers a broad dynamic signal range. The device offers a complete set of stable working low angles, starting from 7°, 12°, 20° and 28° onwards. The model also offers a vertical flow cell where the sample can pass though easily, without any obstacles in its way.

Additionally, the company claims that the PN3621 has the smallest footprint available in the market, saving rare lab space and allowing a flexible use at different locations.

Areas of applications include biotechnology, biopharmaceutical analysis, food and agro science, polymer science, environmental research and nanotechnology.