Saudi Arabia: Pathogens Making Pathogenic Gut Bacteria Feel Uncomfortable
Scientists at the Saudi Arabian Kaust University found a protein in gut-dwelling pathogens that changes shape under human body temperatures. This leads to toxins responsible for diarrheal disease. The researchers want to use this insight to develop drugs that block this reaction.
Thuwal/Saudi Arabia — Pathogenic bacteria come alive at the metabolic level when they enter the warmth of the human gut, firing up genes that encode toxins and other compounds harmful to our bodies. A Kaust-led study shows how a critical bacterial protein senses changes in temperature to slacken DNA strands and boost gene expression in diarrhea-inducing bugs.
Having determined how these bacteria sense that they are inside humans, the scientists could try to conceive of small molecules to perturb this mechanism. Such compounds would block bacteria from adapting to their environment, which would weaken them and facilitate eliminating them.
Gut-dwelling bacteria that cause food-borne illnesses, including Salmonella, disease-causing strains of E. coli and the cholera pathogen Vibrio cholerae, all use a protein called H-NS to condense their DNA and broadly restrict gene expression. Short for histone-like nucleoid-structuring protein, H-NS forms multiunit complexes that help the microbes stay relatively dormant when free-floating in the environment. However, these complexes must break up for the protein to release its grip on DNA, which then allows the bacteria to thrive within a warm-blooded host, explains group leader, Stefan Arold.
With his colleagues Łukasz and Mariusz Jaremko, Arold and research scientist Umar Hameed have now combined structural and biophysical experiments. This has enabled them to identify the exact part of H-NS, dubbed site, that changes its conformation in response to a temperature rise, prompting the protein complexes to fall apart.
Computer simulations, performed with Kaust colleague Xin Gao and Jianing Li, at the University of Vermont, USA, helped Arold’s team deepen these insights. Ultimately, they demonstrated that the partial disassembly of the H-NS complexes leads the protein to adopt a self-inhibiting form that also blocks its ability to bind and recognize DNA.
Arold and his team now hope to develop drugs that can stabilize the connection between proteins at human body temperature and prevent H-NS complexes from fragmenting. “If we can find compounds that reinforce the structure against heat-induced unfolding,” Arold says, “then bacteria would not express toxins anymore; they would remain inoffensive and be generally weakened inside humans.”
References:Shahul Hameed, U.F., Liao, C., Radhakrishnan, A.K., Huser, F., Aljedani, S.S... Arold, S.T. H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing. Nucleic Acids Research 47, 2666-2680 (2019).