Micro Bioreactor System How to Collect Massive Quantities of Cell Culture Data

The “ambr 15” automated micro bioreactor system from Sartorius Stedim Biotech (SSB) combined with a Nova Biomedical (Nova) “Bioprofile Flex2” automated cell culture analyzer enables rapid at-line sample collection and analysis of massive quantities of key cell culture data.

SSB and Nova have collaborated to seamlessly integrate the software interface that controls the ambr 15 workstation for automated parallel processing of 24 or 48 single-use bioreactors (10–15 ml working volume) with the cell culture analysis capabilities of the Bioprofile Flex2. According to SSB, the combined system provides a unique tool to simultaneously run, sample and analyze a massive number of cell culture conditions during cell line and media development by Design of Experiments (DoE).

Combining the two systems enables fully integrated automatic sample transfer, analysis and automated feedback control in each single-use bioreactor. This allows collection of enormous quantities of cell culture data, including total and viable cell density, viability, cell diameter, pH, PCO2, PO2, glucose, lactate, glutamine, glutamate, ammonium, Na+, K+, Ca++ and osmolality, which can be sampled and measured with a complete cycle time of just 6–7 minutes.

The ambr 15 software simultaneously acquires the Bioprofile Flex2 data set and processes it, for example, by performing complex in-run calculations of how much feed volume to automatically add during a run. This saves time by significantly increasing the amount of consistent clone selection, media development and culture parameter data that a scientist can generate per run.

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